This would be a dedicated machine. If the speed of the ethernet
card(s) is significantly higher than the equipment under test,
I don't see how it could be a problem using a single machine.
You may be in for a surprise. Any other traffic running over the same
PCI-bus as your network card or consuming CPU cycles will likely affect
your results negatively. What is the bandwidth of the bus the network
interface sits on, and what devices share that bus?
Even modern hardware can have problems filling a 100mbps link with small
packets. (Too many interrupts to handle.) Certain GbE interfaces
struggles really hard to receive 300 Mbps for any packetsize, and will
definitely not be able to transmit that much.